Assessment of environmental contamination associated with a mammalian cell transformation assay |
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Authors: | E B Sansone A M Losikoff W B Lebherz III J A Poiley |
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Institution: | (1) Environmental Control and Research Laboratory, Frederick Cancer Research Center, P. O. Box B, 21701 Frederick, MD |
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Abstract: | Summary To estimate worker exposures to, and environmental contamination from, test chemicals and organic solvents used in an in vitro
assay to assess the carcinogenic potential of chemicals, sodium fluorescein, a noncarcinogenic fluorescent material, was dissolved
in tissue culture medium used to maintain early passage hamster embryo cells. Personal and environmental samples were taken
over a 14-d period. The assay was performed according to standard procedures in a ventilated glove box or laminar flow safety
cabinet. Considerably more than 99% of the chemical contamination found was recovered from the interiors of the glove box
and hood and from disposable equipment. Contamination outside the containment units (less than 1 μg) resulted from intralaboratory
transport of chemicals, treated cultures, and contaminated equipment. We conclude that the standard operating practices and
procedures provided adequate safeguards for personnel and the environment.
Research sponsored by the National Cancer Institute under Contract N01-CO-75380, with Litton Bionetics, Inc. |
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Keywords: | chemical contamination hazard safety transformation assay |
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