|
|||||
|
|
Lycopene inhibits PDGF-BB-induced retinal pigment epithelial cell migration by suppression of PI3K/Akt and MAPK pathways |
|
| Authors: | Chi-Ming Chan Jia-You Fang Chi-Yea Yang |
| Affiliation: | a School of Medicine, Fu Jen Catholic University, Taipei Hsien, Taiwan, ROC b Department of Ophthalmology, Cardinal Tien Hospital, Taipei Hsien, Taiwan, ROC c Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan, Taiwan, ROC d Department of Biotechnology, Vanung University, Taoyuan, Taiwan, ROC |
| Abstract: | Retinal pigment epithelial (RPE) cells play a dominant role in the development of proliferative vitreoretinopathy (PVR), which is the leading cause of failure in retinal reattachment surgery. Several studies have shown that platelet-derived growth factor (PDGF) exhibits chemotaxis and proliferation effects on RPE cells in PVR. In this study, the inhibitory effect of lycopene on PDGF-BB-induced ARPE19 cell migration is examined. In electric cell-substrate impedance sensing (ECIS) and Transwell migration assays, significant suppression of PDGF-BB-induced ARPE19 cell migration by lycopene is observed. Cell viability assays show no cytotoxicity of lycopene on RPE cells. Lycopene shows no effect on ARPE19 cell adhesion and is found to inhibit PDGF-BB-induced tyrosine phosphorylation and the underlying signaling pathways of PI3K, Akt, ERK and p38 activation. However, PDGF-BB and lycopene show no effects on JNK activation. Taken together, our results demonstrate that lycopene inhibits PDGF-BB-induced ARPE19 cell migration through inhibition of PI3K/Akt, ERK and p38 activation. |
| Keywords: | Lycopene Retinal pigment epithelial cell ARPE19 PDGF Cell migration Cell adhesion Cytotoxicity Proliferative vitreoretinopathy PI3K/Akt MAPK |
| 本文献已被 ScienceDirect 等数据库收录！ | |