ALIX Is Recruited Temporarily into HIV-1 Budding Sites at the End of Gag Assembly |
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| Authors: | Pei-I Ku Mourad Bendjennat Jeff Ballew Michael B Landesman Saveez Saffarian |
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| Institution: | 1. Department of Physics and Astronomy, University of Utah, Salt Lake City, Utah, United States of America.; 2. Center for Cell and Genome Science, University of Utah, Salt Lake City, Utah, United States of America.; 3. Department of Biology, University of Utah, Salt Lake City, Utah, United States of America.; University of Alabama at Birmingham, United States of America, |
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| Abstract: | Polymerization of Gag on the inner leaflet of the plasma membrane drives the assembly of Human Immunodeficiency Virus 1 (HIV-1). Gag recruits components of the endosomal sorting complexes required for transport (ESCRT) to facilitate membrane fission and virion release. ESCRT assembly is initiated by recruitment of ALIX and TSG101/ESCRT-I, which bind directly to the viral Gag protein and then recruit the downstream ESCRT-III and VPS4 factors to complete the budding process. In contrast to previous models, we show that ALIX is recruited transiently at the end of Gag assembly, and that most ALIX molecules are recycled into the cytosol as the virus buds, although a subset remains within the virion. Our experiments imply that ALIX is recruited to the neck of the assembling virion and is mostly recycled after virion release. |
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