Characterisation of alcohol dehydrogenase in Najas marina L. |
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| Affiliation: | 1. National Institute of Ecology, Seo-Cheon Gun, Chungcheongnam Province 325-813, South Korea;2. Wetlands and Birds Korea, Geo-Je Dong, Yeon-Je Gu, Busan 611-071, South Korea;3. Department of Physical & Environmental Sciences, University of Toronto, Toronto, Ontario M1C 1A4, Canada;4. Department of Biological Sciences, Pusan National University, Jang-Jeon Dong, Gum-Jeong Gu, Busan 609-735, South Korea;5. Institute of Environmental Technology & Industry, Pusan National University, Jang-Jeon Dong, Gum-Jeong Gu, Busan 609-735, South Korea;1. Laboratory of Marine Geochemistry, Geoscience Institute, Federal University of Bahia, Rua Barão de Jeremoabo, s/n, 40170-020 Salvador, BA, Brazil;2. Laboratory of Marine Organic Chemistry, Oceanographic Institute, University of São Paulo, Praça do Oceanográfico, 191, 05508-900 São Paulo, SP, Brazil;3. Environmental and Fisheries Sciences Division, Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 2725 Montlake Boulevard East, Seattle, WA 98112, United States;4. Laboratory of Marine Life Ecophysiology, Oceanographic Institute, University of São Paulo, Praça do Oceanográfico, 191, 05508-900 São Paulo, SP, Brazil;5. Laboratory of Reproductive Ecology and Recruitment of Marine Organisms, Oceanographic Institute, University of São Paulo, Praça do Oceanográfico, 191, 05508-900 São Paulo, SP, Brazil |
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| Abstract: | ![]()
In Najas marina L., total alcohol dehydrogenase (ADH) activity is very high in seeds. The pattern is very complex. In mature tissue, the activity is much lower and only one zone of activity is detected. From molecular weight comparisons, densitometric scans and dissociation-reassociation experiments, we could conclude that the major ADH system is controlled by two genes, Adh 1 and Adh 2. The gene product shows interactions and forms homodimers, heterodimers and intergenic heterodimers. Some polymorphism was found within populations of N. marina subsp. marina. In N. marina subsp. intermedia (Gorski) Casper, many different types of zymograms could be detected. Three additional allelic forms were found for Adh 1 and two for Adh 2. In pollen, an additional system is induced which forms heterodimers with Adh 1 and is probably under the control of a third gene, Adh 3. The enzymes are nicotinamide-adenine dinucleotide (NAD) specific and oxidise a wide variety of primary alcohols, with preference for short chain lengths. |
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