广东省水库3株水华微囊藻16s rRNA序列分析

微囊藻是有害淡水水华的主要藻种,但其形态分类困难,制约了相关研究的深入;16SrRNA序列分析广泛应用于微生物种类鉴定。测定广东3个水库的3株水华微囊藻16SrRNA基因部分序列,运用Mega3软件分析其遗传特征,结果表明,3株藻同源序列长度为1305bp,没有插入与缺失,序列中有3个变异位点,A T含量最大差异仅为0.2%,核苷酸相似性在99.85%以上,不能区分广东省水库同一藻种的不同藻株,表明水华微囊藻种内16SrRNA基因序列相当保守。要准确鉴定广东省水库微囊藻种类,丰富广东省水库微囊藻分子层面的分类资料,需要比较更多形态和地理来源的藻株,建立16SrRNA和其它基因序列核苷酸数据库,分析种间和种内差异,设计种专一性的分子探针。

Sequence Analysis of 16s rRNA Gene from 3 Microcystis flos-aquaeaeruginosa in Guangdong Rerservoirs

Microcystis is a freshwater harm algal bloom species,and its taxonomy is difficult to be identified with traditional approach.16S rRNA has been successfully applied to identify this microorganism species.In this study,partial sequences of 16srRNA gene of three strains of M.flos-aquae from 3 reservoirs in Guangdong Province were determined.Clustal1.83 and MEGA3 software package was used to analyze their genetic characteristics.It showed that alignment of the 3 sequences totaled 1035bp without any indels,and nucleotide similarity were above 99.85%,which suggested that discrimination of the 3 strains was failed,and 16S rDNA sequences were quite conservative within species.The degree of nucleotide dissimilarity among endemic Microcystis species are yet to be determined.In order to identify Microcystis species from reservoirs in Guangdong Province,more strains of various morphology and origins should be analyzed to build nucleotide database of sequences of 16s rRNA and other gene,so that difference within and among species could be found and species-specific probes could be designed.