Application of statistically-based experimental designs for the optimization of nisin production from whey |
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Authors: | Liu Chuanbin Liu Yan Liao Wei Wen Zhiyou Chen Shulin |
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Institution: | (1) Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA |
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Abstract: | Statistically-based experimental designs were applied for the optimization of nisin production by Lactococcus lactis in a whey-based medium. Yeast extract, KH2PO4, and MgSO4 were identified to have significant effects on nisin biosynthesis by a Plackett–Burman design. These three significant factors were subsequently optimized using central composite design, and the optimal conditions were determined to be 12.067 g l–1 for yeast extract, 0.569 g l–1 for KH2PO4, and 0.572 g l–1 for MgSO4. The validity of the optimal conditions was verified by a separate experiment. |
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Keywords: | experimental design fermentation nisin optimization whey |
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