RFLP and linkage analysis of the porcine casein loci—CASAS1, CASAS2, CASB and CASK |
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| Authors: | A L Archibald S Coupewhite C S Haley C W Beattie L J Alexander |
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| Institution: | Roslin Institute (Edinburgh) Roslin, Midlothian, EH25 9PS, Scotland. UK;USDA, ARS, NPA, Roman L. Hruska US Meat Animal Research Center, Box 166. Clay Center. Nebraska NE 68933. USA |
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| Abstract: | Restriction fragment length polymorphisms (RFLPs) were revealed at the porcine casein loci with the following combinations of restriction endonucleases and porcine cDNA clones: αs1,-casein (TaqI); αs2-casein (BamHI); and ß-casein (Sacl). These RFLPs were shown to be under simple monogenic control by segregation analysis of two- and three-generation families. The CASAS1, CASAS2 and CASB casein loci were also shown to be linked with no recombinant haplotypes observed amongst 77 meioses in Large White and Meishan F1 and F2 crosses. No recombinants were observed in a further 106 meioses that were informative for linkage between CASAS1 and CASAS2. |
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| Keywords: | pig casein linkage RFLPs |
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