Monovalent carboxylic ionophores inhibit transport of carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells and cause accumulation of enzyme precursor |
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Authors: | Y Kitagawa A Murakami E Sugimoto |
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Institution: | Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan |
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Abstract: | Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degraded slowly with a half-life of more than 16 min. Valinomycin, nonactin, A23187, X-537A (lasalocid), bromo-lasalocid, and carbonyl cyanide m-chlorophenylhydrazone did not exhibit these effects at concentrations at which they did not inhibit protein synthesis of the cells. |
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Keywords: | Carbamoyl-phosphate synthetase I Carboxylic ionophore Monensin Nigericin Reuber hepatoma H-35 Mitochondrial enzyme CPS-I carbamoyl-phosphate synthetase I pCPS-1 precursor for CPS-I Hepes 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid SDS sodium dodecyl sulfate PMSF phenylmethylsulfonyl fluoride |
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