In situ gynogenetic haploid plants of chicory (Cichorium intybus L.) after intergeneric hybridization with Cicerbita alpina Walbr.

The possibility of obtaining haploid plants of chicory (Cichorium intybus L.) was investigated through intergeneric hybridization. Chicory plants (industrial chicory and Chioggia) were pollinated with pollen of Lactuca tatarica L. and Cicerbita alpina Walbr. Many achenes contained embryos which were rescued in vitro. Only a few embryos developed into plants which were then acclimatized in soil. Among them, three expressed a chicory phenorype and were haploidAbbreviations DAPI 4,6-diamidinophenylindole - DH doubled haploid - DNA deoxyribonucleic acid - GUS ß glucuronidase - RFLP Restriction Fragment Length Polymorphism - X-Gluc 5-bromo-4-chloro-3-indolyl-glucuronide - mtDNA mitochondrial DNA