Membrane-bound/soluble IL-2 receptor (IL-2R) and levels of IL-1α, IL-2, and IL-6 in the serum and in the PBMC culture supernatants from 17 patients with hematological malignancies |
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Authors: | Giovanni Mantovani Antonio Maccio Paola Lai Massimo Ghiani Emiliano Turnu G Sergio Del Giacco |
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Institution: | (1) Department of Medical Oncology, University of Cagliari, 09124 Cagliari, Italy |
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Abstract: | The present study investigated the peripheral blood mononuclear cells (PBMC) blastic responses to PHA, PHA plus recombinant
IL-2 (rIL-2) and rIL-2 alone; the expression of membrane-bound IL-2R on PHA-stimulated PBMC; and the levels of IL-1α, IL-2,
IL-6, and sIL-2R in serum and in culture supernatants from PHA-stimulated PBMC in 17 patients with hematological malignancies
(mean age 58.5 yr, range 22–82): 6 with non-Hodgkin’s lymphoma (NHL), 4 with Hodgkin’s lymphoma (HL), 5 with Hairy cell leukemia,
1 with chronic myelogenous leukemia, and 1 with chronic lymphocytic leukemia. The patients with HL and NHL with active disease
(AD) were separated from those in clinical remission. The patients with AD were studied at diagnosis (obviously before therapy)
and the patients in clinical remission were out of therapy since at least 6 mo. The lymphocyte blastogenic response to PHA
was significantly lower in patients with HL and NHL with AD than in the control group. The response to rIL-2 alone was in
the same range in the control group and in HL and NHL AD patients. By adding rIL-2 to PHA there was an increase of the blastogenic
response of the same patients. The percentage of CD25 expressed on PHA-stimulated lymphocytes from patients with HL and NHL
AD and from normal subjects is in the same range. Serum levels of IL-2, IL-6, and sIL-2R were significantly higher in HL and
NHL AD patients than in controls as well as in all other hematological malignancies. Supernatants derived from PHA-stimulated
PBMC were assessed for the presence of cytokines and sIL-2R by ELISA. The levels of IL-2, IL-6, and sIL-2R were significantly
lower in HL and NHL AD patients than in controls as well as in all other hematological malignancies. |
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Keywords: | Hematological malignancies interleukin-1 interleukin-2 interleukin-2 receptor interleukin-6 peripheral blood mononuclear cells soluble interleukin-2 receptor |
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