Carbon oxidation and incorporation patterns in batch cultures ofMethylomonas L3 |
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Authors: | I-Ming Chu Eleftherios T Papoutsakis |
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Institution: | (1) Department of Chemical Engineering, Rice University, P.O. Box 1892, 77251 Houston, Texas, USA |
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Abstract: | Summary A recently developed methodology of directly measuring the oxidation and incorporation patterns of carbon substrate in continuous cultures of RuMP-type methylotrophs is extended to batch cultures of the obligate methylotrophMethylomonas L3. The ratio of cyclic to total substrate oxidation varies with the initial methanol concentration from 0 to 68%. Formaldehyde, as a methanol cosubstrate, enhances the net substrate oxidation. The substrate oxidation and incorporation pattern is also affected by the state of the culture inoculum. |
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