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Electron-microscopic immunocytochemical demonstration of separate vasotocinergic,mesotocinergic and somatostatinergic neurons in the hypothalamic magnocellular preoptic nucleus of the frog
Authors:Mrs J Van Vossel-Daeninck  K Dierickx  F Vandesande  A Van Vossel
Institution:(1) Department of Embryology and Comparative Histology, Rijksuniversiteit Gent, Belgium;(2) Laboratorium voor Embryologie en Vergelijkende Histologie, Rijksuniversiteit-Godshuizenlaan 4, B-9000 Gent, Belgium
Abstract:Summary The hypothalamic magnocellular preoptic nucleus of Rana temporaria was studied at the electron-microscopic level with the use of the unlabeled antibody peroxidase-antiperoxidase complex (PAP) technique. The magnocellular preoptic nucleus of R. temporaria contains at least three different types of neurons: (1) Vasotocinergic neurons, (2) mesotocinergic neurons, and (3) neurons that contain either somatostatin or an immunologically related peptide. The present results are in complete agreement with those of previous immunocytochemical studies conducted at the light microscopic level.
Keywords:Hypothalamus  Rana temporaria  Vasotocinergic neurons  Mesotocinergic neurons  Somatostatinergic neurons  Electron microscopic immunocytochemistry
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