Isolation and characterization of cDNA clones encoding ADP-glucose pyrophosphorylase (AGPase) large and small subunits from chickpea (Cicer arietinum L.) |
| |
Authors: | Singh Salvinder Choi Sang-Bong Modi Mahendra K Okita Thomas W |
| |
Institution: | Institute of Biological Chemistry, Washington State University, PO Box 646340, Pullman, WA 99164-6340, USA. |
| |
Abstract: | Four cDNA clones encoding two large subunits and two small subunits of the starch regulatory enzyme ADP-glucose pyrophosphorylase (AGPase) were isolated from a chickpea (Cicer arietinum L.) stem cDNA library. DNA sequence and Southern blot analyses of these clones, designated CagpL1, CagpL2 (large subunits) and CagpS1 and CagpS2 (small subunits), revealed that these isoforms represented different AGPase large and small subunits. RNA expression analysis indicated that CagpL1 was expressed strongly in leaves with reduced expression in the stem. No detectable expression was observed in seeds and roots. CagpL2 was expressed moderately in seeds followed by weak expression in leaves, stems and roots. Similar analysis showed that CagpS1 and CagpS2 displayed a spatial expression pattern similar to that observed for CagpL2 with the exception that CagpS1 showed a much higher expression in seeds than CagpS2. The spatial expression patterns of these different AGPase subunit sequences indicate that different AGPase isoforms are used to control starch biosynthesis in different organs during chickpea development. |
| |
Keywords: | AGPase ADP-glucose pyrophosphorylase AMP adenosine 5′-monophosphate PGA 3-phosphoglycerate Pi inorganic phosphate CagpL1 and CagpL2 chickpea AGPase large subunits 1 and 2 CagpS1 and CagpS2 chickpea AGPase small subunits 1 and 2 |
本文献已被 ScienceDirect PubMed 等数据库收录! |