Genetic Mapping and QTL Analysis of Growth-Related Traits in the Pacific Oyster |
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Authors: | Xiang Guo Qi Li Qing Z Wang Ling F Kong |
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Institution: | (1) College of Fisheries, Ocean University of China, Qingdao, 266003, China; |
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Abstract: | The Pacific oyster (Crassostrea gigas) is one of the most important oysters cultured worldwide. To analyze the oyster genome and dissect growth-related traits,
we constructed a sex-averaged linkage map by combining 64 genomic simple sequence repeats, 42 expressed sequence tag-derived
SSRs, and 320 amplified fragment length polymorphism markers in an F1 full-sib family. A total of 426 markers were assigned to 11 linkage groups, spanning 558.2 cM with an average interval of
1.3 cM and 94.7% of genome coverage. Segregation distortion was significant for 18.8% of the markers (P < 0.05), and distorted markers tended to occur on some genetic regions or linkage groups. Most growth-related quantitative
traits were highly significantly (P < 0.01) correlated, and principal component analysis obtained four principal components. Quantitative trait locus (QTL) analysis
identified three significant QTLs for two principal components, which explained 0.6–13.9% of the phenotypic variation. One
QTL for sex was detected on linkage group 6, and the inheritabilities of sex for parental alleles and maternal alleles on
that locus C15 are 39.8% and 0.01%, respectively. The constructed linkage map and determined QTLs can provide a tool for further
genetic analysis of the traits and be potential for marker-assisted selection in C. gigas breeding. |
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