Sugar and Light Effects on the Condition of the Photosynthetic Apparatus of Arabidopsis thaliana Cultured in vitro |
| |
Authors: | Aleksandra Eckstein Patrycja Zi?ba Halina Gabry? |
| |
Institution: | (1) Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krak?w, Poland; |
| |
Abstract: | Light and sugars are fundamental elements of plant metabolism and play signaling roles in many processes. They are also critical
factors determining the condition of plants cultured in vitro. The aim of this work was to investigate the simultaneous influence
of irradiance and sugar content in the medium on the growth and photosynthetic apparatus condition of Arabidopsis thaliana in vitro. Plants were grown on media containing 1 or 3% of sucrose or glucose at three irradiances: 25, 100, and 250 μmol m−2 s−1 (weak, medium, and strong light). Media without sugar were used for control plants. Plant growth parameters were measured
and the following physiological processes were investigated: photosynthesis, blue light-induced chloroplast relocations, and
xanthophyll cycle activity. The expression of genes related to these processes was analyzed. The presence of sugar in the
medium was found to be essential for the growth of Arabidopsis in vitro. Weak light significantly limited growth and the capacity to acclimate to changing light conditions. Strong light
was a source of stress in some cases. Contrary to earlier reports, exogenous sugars showed a positive effect on photosynthesis.
At higher concentration they acted as photoprotectants, overcoming the negative influence of strong light on photosynthesis
and the xanthophyll cycle. The expression of all investigated genes was influenced by irradiance and sugar presence. In many
cases differential effects of sugar type and concentration could be observed. The specific effects of some irradiance/sugar
concentration combinations point to possible interactions between sugar- and light-induced signaling pathways. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|