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A simple method to estimate the percentage of hybridity in canola (Brassica napus) F1 hybrids
Authors:Philip Marshall  Marie-Claude Marchand  Zenon Lisieczko  Benoit S. Landry
Affiliation:(1) Station de recherche Agriculture Canada, 430 Boul. Gouin, J3B 3E6 St-Jean-sur-le-Richelieu, Québec, Canada;(2) King Agro, Research Center, Highway 23 south, R.R #2, N4W 3G7 Listowel, Ontario, Canada
Abstract:We have developed an efficient PCR-based system that uses RAPD markers for the certification of F1 hybrids of canola. These markers were selected by screening five parental lines used in three crosses X, Y and Z with 131, 131 and 322 primers respectively. Stable DNA fragments that were homozygous and specific to the male inbreds were used to certify F1 hybrid populations. The hybrid production system was based on self-incompatibility (SI) alleles that prevent self-pollination of the female parent. The efficiency of two S-alleles was compared under both field and greenhouse conditions. The percentage of hybridity was estimated in different F1 populations. We found a significant difference between the two alleles for their efficiency in controlling selfing; both alleles were stable under greenhouse conditions, one allele appeared less reliable under field conditions.
Keywords:Polymerase chain reaction  Random amplified polymorphic DNA  Self-incompatibility  Brassica campestris  Brassica napus
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