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rDNA replication in a synchronized culture of Saccharomyces cerevisiae
Authors:G M Gimmler  E Schweizer
Affiliation:1. a Division of Medical Toxicology, Ronald O. Perelman Department of Emergency Medicine, New York University School of Medicine, NYU Langone Health, New York, NY;2. a Division of Medical Toxicology, Ronald O. Perelman Department of Emergency Medicine, New York University School of Medicine, NYU Langone Health, New York, NY
Abstract:From a synchronized culture of Saccharomyces cerevisiae role=presentation style=font-size: 90%; display: inline-block; position: relative;>Saccharomyces cerevisiae cells were harvested at different intervals during S-phase. The purified DNA of each sample was challenged with ribosomal RNA by specific DNA/rRNA hybridization. From the constant rDNA/DNA ratio observed it is concluded that in yeast, the replication of the rRNA cistrons is not restricted to a distinct interval but proceeds at a constant rate throughout the entire S-period.
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