| 小麦叶绿体中细胞分裂素结合蛋白的功能 |
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| 引用本文： | 张华敏,刘愚,沈允钢.小麦叶绿体中细胞分裂素结合蛋白的功能[J].植物生理与分子生物学学报,1994(4). |
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| 作者姓名： | 张华敏 刘愚 沈允钢 |
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| 作者单位： | 中国科学院上海植物生理研究所 |
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| 基金项目： | 中国科学院重点基金,国家自然科学重点基金 |
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| 摘 要： | 采用ＢＡ－Ｓｅｐｈａｒｏｓｅ６Ｂ亲和层析法从小麦叶绿体中纯化了ＣＴＫ结合蛋白并制备了抗体。小麦叶绿体经ＣＴＫ结合蛋白的抗体处理后，表现出：希尔反应活力降低；ＭＶ（包括光系统Ｉ和Ⅱ）和ＰＭＳ（包括光系统Ｉ）系统的毫秒延迟发光的慢相强度下降；光合磷酸化活力受到抑制。说明小麦叶绿体中的ＣＴＫ结合蛋白存在状态的改变会影响叶绿体的能量转换过程。
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| 关 键 词： | CTK结合蛋白，抗体，希尔反应，毫秒延迟发光，光合磷酸化 |
Study on the Function of Cytokinin-Binding Protein in Wheat Chloroplasts |
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| ZHANG Hua-Min,LIU Yu and SHEN Yun-Gang.Study on the Function of Cytokinin-Binding Protein in Wheat Chloroplasts[J].Journal Of Plant Physiology and Molecular Biology,1994(4). |
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| Authors: | ZHANG Hua-Min LIU Yu and SHEN Yun-Gang |
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| Abstract: | Cytokinin-binding protein (CBP) was isolated from chloroplasts of wheat leaves and purified to a single protein by affinity chromatography on a BAsepharose 6B column and then used as antigen. The rabbit antisera prepared contained antibodies against the CBP as judged by double--immuno--diffusion test. When chloroplasts were treated with the antiserum against CBP, Hill reaction activity was inhibited and the intensity of the slow phase of millisecond delayed light emission (msDLE) was reduced. The activity of photophosphorylation was also remarkably inhibited. It is suggested that CBP may affect the energy transduction process of chloroplasts. |
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| Keywords: | CTK--binding protein antiserum Hill reaction msDLE photophosphorylation |
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