Rhizoferrin — a novel siderophore from the fungusRhizopus microsporus var.rhizopodiformis |
| |
Authors: | Hartmut Drechsel Jörg Metzger Stefan Freund Günther Jung Johan R Boelaert Günther Winkelmann |
| |
Institution: | (1) Institut für Organische Chemie, Universität Tübingen, Auf der Morgenstelle 18, W-7400 Tübingen, Germany;(2) Unit for Renal and Infectious Diseases, Algemeen Ziekenhuis St Jan, Brugge, Belgium;(3) Mikrobiologie/Biotechnologie, Universität Tübingen, Auf der Morgenstelle 1, W-7400 Tübingen, Germany |
| |
Abstract: | Summary From a strain ofRhizopus microsporus var.rhizopodiformis a novel siderophore, named rhizoferrin, was isolated by ion-exchange column chromatography, gel filtration and preparative HPLC. Hydrolysis with 6 M HCl and subsequent gas chromatography/mass spectrometry (GUMS) of the esterified/trifluoroacetylated derivatives indicated that citric acid and diaminobutane were the only constituents. From positive fastatom-bombardment (FAB) and ion-spray tandem mass spectrometry, a molecular mass of 436 Da and the assignment of several daughter ion fragments could be obtained, which indicated the presence of two citric acid residues and one diaminobutane residue. NMR studies finally confirmedN
1,N
4-bis(1-oxo-3-hydroxy-3,4-dicarboxybutyl)-diaminobutane as the structure of rhizoferrin. The iron-binding property was demonstrated on chromeazurol S plates and its siderophore activity was confirmed by iron transport measurements in young mycelia ofR. microsporus. While rhizoferrin and also ferrioxamines B and E proved to be effective siderophores, coprogen was a poor siderophore in this fungus. |
| |
Keywords: | Rhizoferrin Rhizopus Siderophore Iron transport |
本文献已被 SpringerLink 等数据库收录! |
|