Quantification of 1,5-anhydro-d-glucitol in urine by automated borate complex anion-exchange chromatography with an immobilized enzyme reactor |
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| Institution: | 1. Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co., 239, Iwahana-machi, Takasaki-shi, Gunma 370-12, Japan;2. Takasaki Research Laboratories, Nippon Kayaku Co., 219, Iwahana-machi, Takasaki-shi, Gunma 370-12, Japan;3. Diagnostic Agent Development Office, Nippon Kayaku Co., 11–12, Fujimi 1-chome, Chiyoda-ku, Tokyo 102, Japan;1. Department of Chemistry, Michigan State University, East Lansing, MI 48824, United States;2. Department of Chemistry, Binghamton University, Binghamton, NY 13902, United States;1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan;2. Center for Atomic and Molecular Technologies, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan;1. Research Institute of Translational Medicine, The First Bethune Hospital of Jilin University, Changchun, 130061, PR China;2. Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun, 130012, PR China;3. School of Life Sciences, Northeast Normal University, Changchun, 130024, PR China;4. Beijing Institute of Drug Metabolism, Beijing, 102209, PR China;1. Laboratory of Arctic Mineralogy and Material Sciences, Kola Science Centre, Russian Academy of Sciences, 14 Fersman Str, Apatity, 184209, Russia;2. Grebenshchikov Institute of Silicate Chemistry, Makarov Emb, St. Petersburg, 199053, Russia;3. Department of Chemistry, Lomonosov Moscow State University, 119991, Moscow, Russia;4. Institute of Chemistry, Saint Petersburg State University, Ulianovskaya 5, St. Petersburg, 198504, Russia;5. Department of Crystallography, Saint Petersburg State University, University Emb, St. Petersburg, 199034, Russia;6. Geological Institute, Kola Science Centre, Russian Academy of Sciences, 14 Fersman Street, Apatity, 184209, Russia |
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| Abstract: | HPLC using a borate form of a strongly anion-exchange resin column and an immobilized enzyme reactor for colorimetric detection was used to quantify urinary 1,5-anhydro-d-glucitol. Urine samples were introduced into the system every 7 min without any pretreatment, and after separation of interfering substances in the column, 1,5-anhydro-d-glucitol was successively detected. Quantitative determination of urinary 1,5-anhydro-d-glucitol was possible within the 1.2–300 μmol/l range. The coefficient of variance was less than 3% and the correlation between results obtained with our system (y) and those obtained by gas chromatography–mass spectrometry (x) was y=0.983x−1.287 μmol/l (n=42, r=0.998). |
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