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| H.pylori-L型感染及OPN表达与胃癌浸润转移的相关性研究 | |
| 引用本文： | 俞岚,周蕾,于东红,甘怀勇,唐素兰. H.pylori-L型感染及OPN表达与胃癌浸润转移的相关性研究[J]. 中国微生态学杂志, 2011, 23(9): 802-805,809 |
| 作者姓名： | 俞岚 周蕾 于东红 甘怀勇 唐素兰 |
| 作者单位： | 1. 蚌埠医学院第一附属医院病理科,蚌埠医学院病理学教研室,安徽省感染与免疫重点实验室,安徽蚌埠233030 2. 蚌埠医学院病原生物学教研室,安徽蚌埠,233030 |
| 基金项目： | 安徽省高校青年教师资助计划 |
| 摘 要： | 目的通过检测胃癌中幽门螺杆菌L型（Helicobacter pylori L-form,H.pylori-L型）感染以及骨桥蛋白（osteopontin,OPN）的表达情况,探讨H.pylori-L型在胃癌侵袭、转移中的作用。方法将胃癌BGC-823细胞与H.pylori-L型按不同比例共培养,细胞爬片进行免疫组化（SP法）检测BGC-823细胞OPN的表达情况。革兰染色及免疫组化检测120例胃癌组织中H.pylori-L型的感染情况。免疫组化检测胃癌组织中的OPN的表达。结果 （1）随着细菌比例增大,细胞爬片OPN阳性的胃癌BGC-823细胞数逐渐增加,各组之间差异有统计学意义（F=137.5,P〈0.01）。（2）胃癌组织H.pylori-L型阳性组的OPN表达阳性率为70.73%,显著高于H.pylori-L型阴性组OPN表达阳性率42.1%（P〈0.005）。胃癌中H.pylori-L型阳性率与肿瘤的大小、癌细胞的血管侵袭、浸润深度及淋巴结转移具有相关性（P〈0.001～P〈0.05）。（3）胃癌组织中H.pylori-L型阳性率与OPN的阳性表达呈正相关性（r=0.27,P〈0.001）。结论 H.pylori-L型感染增强了胃癌细胞的侵袭和转移能力,其机制可能与胃癌细胞的OPN表达量增加有关。 |
| 关 键 词： | 幽门螺杆菌L型 胃癌 侵袭 转移 OPN 免疫组织化学 |
Relationships between Helicobacter pylori L-forms infection and expression of OPN and invasion and metastasis of gastric carcinoma |
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| YU Lan,ZHOU Lei,YU Dong-hong,GAN Huai-yong,TANG Su-lan. Relationships between Helicobacter pylori L-forms infection and expression of OPN and invasion and metastasis of gastric carcinoma[J]. Chinese Journal of Microecology, 2011, 23(9): 802-805,809 | |
| Authors: | YU Lan ZHOU Lei YU Dong-hong GAN Huai-yong TANG Su-lan |
| Affiliation: | YU Lan1,ZHOU Lei1,YU Dong-hong1,GAN Huai-yong1,TANG Su-lan2(1.Department of Pathology,Bengbu Medical College,the First Affiliated Hospital of Bengbu Medical Collge,Anhui Key Laboratory of Infection and Immunology,Bengbu 233030,China,2.Department of Microbiology,China) |
| Abstract: | Objective To detect Helicobacter pylori L-form(H.pylori-L) infection and the expression of OPN in gastric carcinoma,and discuss the role of H.pylori-L infection in the progression of gastric carcinoma.Method Gastric cancer BGC-823 cells were co-incubated with H.pylori-L in different proportions;The expressions of OPN in BGC-823 cells were assessed by immunohistochemical staining.H.pylori-L infection and OPN expression were detected by Gram's staining and immunohistochemical staining in 120 cases of gastric ... |
| Keywords: | Helicobacter pylori L-forms Gastric carcinoma Invasion Metastasis OPN Immunohistochemistry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |