Expression of human interleukin-2 receptor cDNA in E. coli |
| |
| Authors: | P K Chanda G F Chen Y Baine W J Leonard W C Greene T W Chang N T Chang |
| |
| Affiliation: | 1. Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA;2. Center for Biostatistics, The Ohio State University, Columbus, Ohio, USA;3. Division of Hematology, Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, Ohio, USA;4. Nationwide Children''s Hospital, Columbus, Ohio, USA |
| |
| Abstract: | cDNAs for human interleukin-2 receptor were recently cloned and sequenced (Leonard et al., 1984, Nature 311, 626-631; Nikaido et al., 1984, Nature 311, 631-635; Cosman et al., Nature 312, 768-771). In the studies reported here, we describe the expression of a cDNA clone for the human interleukin-2 receptor in E. coli using an "open reading frame" expression vector pMR100. The inserted cDNA was expressed in E. coli transformants as a tripartite fusion polypeptide fused to the lambda cI protein at its amino terminus and to beta-galactosidase at its carboxy terminus. We demonstrate that the bacterially produced IL-2 receptor protein can bind to IL-2. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
