Immunocytochemical localization of the Ca2+-ATPase polypeptide in human platelets |
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Authors: | G H Herbener W L Dean |
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Institution: | Department of Anatomical Sciences, University of Louisville School of Medicine, KY 40292. |
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Abstract: | Specific polyclonal antibodies raised against purified human platelet Ca2+-ATPase were used with protein A-gold immunocytochemistry to localize this protein in human platelets. Immunolabeling specifically detected Ca2+-ATPase over the surface connected membrane system (SCS) in sections of paraformaldehyde-fixed, Lowicryl-embedded platelets. The maximum density of label, determined by quantitative morphometric techniques, was observed over electron-dense regions within the SCS which may represent specialized structures for uptake and release of Ca2+. Less intense immunolabeling was observed over cytosol and may represent localization over the dense tubular system (DTS) which was not readily visualized under the processing procedures employed. |
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