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In vitro organogenesis from root culture segments of Bixa orellana L. (Bixaceae) |
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| Authors: | Ana Claudia Ferreira da Cruz Diego Ismael Rocha Lourdes Iarema Marília Contim Ventrella Marcio Gilberto Cardoso Costa Vespasiano Borges Paiva Neto Wagner Campos Otoni |
| Affiliation: | 1. Departmento de Biologia Vegetal, Laboratório de Cultura de Tecidos/BIOAGRO, Universidade Federal de Vi?osa, Campus Universitário, Av. P.H. Rolfs, 36570-000, Vi?osa, MG, Brazil 2. Departmento de Biologia Vegetal, Laboratório de Anatomia Vegetal, Universidade Federal de Vi?osa, Campus Universitário, Av. P.H. Rolfs, 36570-000, Vi?osa, MG, Brazil 3. Departamento de Ciências Biológicas, Centro de Biotecnologia e Genética, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, Salobrinho, 45662-000, Ilhéus, BA, Brazil 4. Universidade Federal do Mato Grosso do Sul, Centro de Ciências Agrárias, Campus Universitário de Chapad?o do Sul, 79560-000, Chapad?o do Sul, MS, Brazil 5. Plant Biology Department/BIOAGRO, Federal University of Vi?osa, P.H. Rolfs Avenue, s/n, University Campus, 36570-000, Vi?osa, MG, Brazil |
| Abstract: | The organogenic potential of root explants derived from cultured seedlings of Bixa orellana L. (annatto) was investigated in response to different incubation conditions and either 4.44 μM 6-benzyladenine, 4.54 μM Thidiazuron, or 4.56 μM Zeatin. Explants cultured in liquid media with agitation generally showed better development of adventitious buds versus explants cultured on semi-solid media. The most adventitious buds developed from explants cultured in liquid media under a 16-h photoperiod. Use of Zeatin and Thidiazuron promoted the development of more adventitious buds than 6-benzyladenine but morphological abnormalities among regenerating shoots and plants were observed. Fewer adventitious buds developed from explants cultured in liquid media supplemented with 6-benzyladenine, but the buds gave rise to the highest percentage of morphologically normal regenerated plants. Histological analyses showed that adventitious buds originated from cell proliferation within the pericycle, opposite the protoxylem poles of the explant. Seedling root tissue is useful for in vitro propagation of B. orellana. |
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