Glucose biosensor based on nanohybrid material of gold nanoparticles and glucose oxidase on a bioplatform |
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Authors: | Zhang Yan Jia Wenjuan Cui Miao Dong Chuan Shuang Shaomin Kwan Yuen Choi Martin M F |
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Institution: | School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, PR China. |
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Abstract: | A simple and relatively cheap glucose biosensor based on a combination of gold nanoparticles (Au NPs) and glucose oxidase (GO(x) ) immobilized on a bioplatform eggshell membrane was established. Scanning electron microscopy showed successful immobilization of Au NPs/GO(x) on the eggshell membrane. The effects of pH, phosphate buffer concentration, and temperature on the glucose biosensor were studied in detail. The biosensor shows a linear response at a glucose concentration range of 5-525 μM. The detection limit of the biosensor is 2.5 μM (S/N = 3). The biosensor exhibits good repeatability with RSD = 3.6% (n = 6), good operational stability with over 300 measurements and long-term storage stability with a shelf life of at least 6 months. The response time is less than 60 s. The glucose level in commercial food samples has been successfully determined. The proposed work shows potential to develop cost-effective biosensors for biotechnological, biomedical and industrial use. |
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Keywords: | Biosensors Eggshell membrane Enzymes Gold nanoparticles Glucose oxidase |
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