<Emphasis Type="Italic">Drosophila</Emphasis> CENP-C is essential for centromere identity |
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Authors: | Bernardo?Orr Email author" target="_blank">Claudio?E?SunkelEmail author |
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Institution: | 1.Instituto de Biologia Molecular e Celular,Universidade do Porto,Porto,Portugal;2.ICBAS, Instituto de Ciências Biomédicas de Abel Salazar,Universidade do Porto,Porto,Portugal |
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Abstract: | Centromeres are specialized chromosomal domains that direct mitotic kinetochore assembly and are defined by the presence of
CENP-A (CID in Drosophila) and CENP-C. While the role of CENP-A appears to be highly conserved, functional studies in different organisms suggest that
the precise role of CENP-C in kinetochore assembly is still under debate. Previous studies in vertebrate cells have shown
that CENP-C inactivation causes mitotic delay, chromosome missegregation, and apoptosis; however, in Drosophila, the role of CENP-C is not well-defined. We have used RNA interference depletion in S2 cells to address this question and
we find that depletion of CENP-C causes a kinetochore null phenotype, and consequently, the spindle checkpoint, kinetochore–microtubule
interactions, and spindle size are severely misregulated. Importantly, we show that CENP-C is required for centromere identity
as CID, MEI-S332, and chromosomal passenger proteins fail to localize in CENP-C depleted cells, suggesting a tight communication
between the inner kinetochore proteins and centromeres. We suggest that CENP-C might fulfill the structural roles of the human
centromere-associated proteins not identified in Drosophila. |
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