Pokemon、P14ARF 蛋白在食管鳞状细胞癌中的表达及临床意义

目的:探讨食管鳞癌(esophageal squamous cell cacinoma,ESCC)组织中Pokemon和P14ARF表达的关系及其对ESCC临床病理特征和预后的判定价值。方法:应用SP免疫组织化学方法检测Pokemon和P14ARF二种蛋白在96例ESCC组织及20例癌旁正常组织中的表达,分析它们与ESCC病理学特征的关系,以及Pokemon和P14ARF的相关性,并结合随访资料观察上述蛋白表达对ESCC长期预后的影响。结果:癌旁正常组织中未见Pokemon蛋白表达,P14ARF蛋白阳性表达率为90.0%;在ESCC组织中Pokemon和P14ARF阳性表达率分别为75.0%和30.2%。Pokemon表达与P14ARF表达呈负相关(r=-0.458,P=0.000)。Pokemon和P14ARF的表达与TNM分期和淋巴结转移相关(P<0.05)。Pokemon表达阳性组的5年生存率分别为20.8%,显著低于阴性组(P=0.004);P14ARF表达阳性组的5年生存率为41.4%,显著高于阴性组(P=0.011)。结论:Pokemon蛋白的高表达和P14ARF蛋白低表达可能与ESCC的发生、发展关系密切,它们对于ESCC预后评估有一定的临床意义。

Expression and Clinical Significance of Pokemon and P14ARF in Esophageal Squamous Cell Cancer

Objective: To investigate the correlation of the expression of Pokemon and P14ARF in esophageal squamous cell caci-noma(ESCC)tissues,and their values for determining the clinicopathological characteristics and prognosis of ESCC.Methods: Immuno histochemical SP staining was used to detect the expressions of pokemon and P14ARF proteins in 96 ESCC tumor tissues and 20 adjacent normal tissues.Their relationship with the pathological characteristics of ESCC was analyzed.The correlation of abnormal expressions of pokemon and P14ARF was analyzed.The effects of the expressions of the two proteins on the long-term prognosis of ESCC patients were observed based on the follow-up data.Results: In normal tissues there was no Pokemon expression but the positive rate of P14ARF ex-pression was 90.0 %.In ESCC tissues,the positive rate of Pokemon and P14ARF protein were 75 % and 30.2 %,respectively.The ex-pression of Pokemon had negative correlation with expression of P14ARF(r=-0.458,P=0.000).The expression of Pokemon and P14ARF were associated with TNM staging and lymph node metastasis(P<0.05).In Pokemon positive patients,the five-year survival rate was 20.8 %,which was significantly lower than that in Pokemon negative patients(P=0.004).In contrast,the five-year survival rate was 41.4 % in patients with positive expression of P14ARF,which was significantly higher than that in patients with negative expression of P14ARF(P=0.011).Conclusion: The over expression of Pokemon and the less expression of P14ARF maybe play important roles in the develop-ment of ESCC.Pokemon and P14ARF have a certain clinical significance for evaluating the prognosis of ESCC patients.