慢性阻塞性肺疾病大鼠模型中痩素及白介素-8 的表达分析

目的:观察COPD大鼠模型中瘦素(leptin)、白细胞介素8(IL-8)的表达情况,分析其相关性,探讨leptin在COPD发生发展中的作用及意义.方法:36只雄性SD大鼠随机分为①健康对照组；②COPD模型1组:分别于第(1、14)d经气管内注入内毒素200ug,熏5％香烟(第1、14d除外),2h/d,共4周；③COPD模型2组:单纯熏5％香烟2h/d,共12周.观察肺组织病理变化,免疫组化法测定leptin、IL-8在支气管肺组织的表达情况,放免法测定血清leptin及IL-8浓度.结果:细胞因子leptin及炎性因子IL-8在支气管肺组织阳性表达.LPS联合熏烟诱导COPD1组支气管肺组织中leptin(52.67±04.72)和IL-8(59.56± 3.94)表达较单纯熏烟诱导COPD2组leptin(38.89± 2.57)和IL-8(55.22± 3.42)表达明显升高,P＜0.05,两组COPD大鼠模型支气管肺组织中leptin及IL-8表达较正常对照组leptin( 1690± 1.52)和IL-8 (28.00± 4.24)表达均明显增高,P＜0.05,COPD1组血清中leptin(3.26± 0.95)ng/mL和IL-8( 107.51±13.38 )pg/mL-较COPD2组中leptin(2.42± 0.69 )ng/mL和IL-8((94.07± 11.20)pg/mL明显增高,P＜0.05,两组COPD大鼠模型血清中leptin及IL-8浓度较正常对照组leptin(0.95±0.56)ng/mL和IL-8( 39.48± 6.35 )pg/mL浓度显著升高,P＜0.05.血清中Leptin与IL-8表达水平呈显著正相关(r值分别为0.72 0.67 0.84均P＜0.05).经过q检验,两两之间比较均有统计学意义.结论:leptin和IL-8均参与COPD炎症反应过程,并且具有相关性,LPS促进二者的表达.

The Analysis of Leptin and Interleukin-8 Expression in Rat Models ofChronic Obstructive Pulmonary Disease

Objective: To investigate the expression matter of leptin and IL-8 in rat models of COPD,and analyse their relevance,investigate the value of leptin in the development of COPD.Methods: 36 Male SD rats were randomly divided into 3 groups:(1) Control group: untreated rats;(2)COPD1: rats were intratracheally administered LPS(200 μg) on d 1 and 14 and exposed to 5% smoke for 2 h daily for 4 consecutive weeks;(3) COPD2: rats were exposed to 5% smoke for 2 h daily for 12 consecutive weeks.To observe the pathological changes of the lung tissues,and take immunohistochemistry to determine leptin and IL-8 levels expressed in bronchial lung tissue.Serum leptin and IL-8 concentration was examined by radio-immunity method.Results: The cell factor leptin and inflammatory factor IL-8 in bronchial lung tissue were positive expressed.The expression of leptin(52.67±04.72)and IL-8(59.56±3.94) in COPD 1 group was higher than that in COPD2 group leptin(38.89±2.57) and IL-8(55.22±3.42),P<0.05;Both leptin and IL-8 expression in two COPD model groups were higher than that in the normal group(16.90±1.52) and(28.00±4.24),P<0.05;In the COPD1 group,serum leptin level(3.26±0.95) ng/mL and IL-8(107.51±13.38) levels were markedly higher than that in the COPD2 group(2.42±0.69)ng/ml and(94.07±11.20) pg/mL,P < 0.05,and these levels in both COPD groups increased dramatically compared with that in controls(0.95±0.56)ng/mL and IL-8(39.48±6.35) pg/mL,P<0.05.There was negative correlation between leptin and IL-8 expression(r=0.72 0.67 0.84 all P<0.05)in blood.The comparison between the two groups had statistical significance after inspection.Conclusion: Leptin and IL-8 play important roles in the occurrence and development of COPD,which is correlated with each other.In addition,LPS facilitates the expression of leptin and IL-8.