不同临床类型乙型病毒感染者外周血T 细胞亚群与血清HBV DNA 载量 及HBeAg 滴度相关性分析

目的:探讨慢性乙型肝炎病毒(HBV)感染患者外周血T细胞亚群与血清HBV DNA载量及HbeAg滴度的关系。方法:选取103名HBV感染患者和20名健康者为研究对象。流式细胞术检测外周血T细胞亚群,聚合酶链式反应及酶免疫分析法分别检测血清HBV DNA载量及HbeAg滴度。结果:慢性乙型肝炎患者和慢性HBV携带者外周血CD3+T、CD4+T淋巴细胞亚群百分数低于健康对照组,结果有统计学意义(P<0.05或0.01;而CD8+T细胞亚群则呈现相反趋势,结果亦有统计学意义(P<0.05或0.01)。HBeAg阴性组中,HBVDNA水平与CD8+T细胞亚群百分数呈正相关(r=0.567,P<0.01),与CD4+/CD8+T细胞亚群百分数比值呈负相关(r=-0.601,P<0.01),而与CD3+T、CD4+T细胞亚群百分数无相关性。HBeAg阳性组中,HBV DNA水平及HbeAg滴度与CD3+T、CD4+T、CD8+T细胞百分数及CD4+/CD8+T细胞百分数均无相关性(P>0.05)。结论:不同临床类型的慢性乙型肝炎病毒感染患者外周血T细胞亚群存在不同程度细胞免疫功能降低和细胞免疫调节异常。HbeAg阴性的HBV感染患者,其血清HBV DNA水平与外周血T淋巴细胞免疫存在相关性。

Correlation of T-lymphocyte Subsets with Serum HBV DNA Loads and HBeAg Title among Different Clinical Types of Hepatitis B Patients

Objective: To investigate the relationship between T-lymphocyte subsets and HBV DNA levels and serum hepatitis B e-antigen(HBeAg) status in patients with chronic hepatitis B virus(HBV) infection.Methods: 103 patients with chronic HBV infection and 20 healthy blood donors were included in the study.The peripheral T-lymphocyte subsets in these patients were determined by flow cytometry.The serum HBVDNA levels were assessed by polymerase chain reaction(PCR) and HBeAg status were tested by enzyme immunoassay.Results: The percentages of CD3+,CD4+T lymphocytes in peripheral blood and CD4+/CD8+ ratios in Chronic Hepatitis B(CHB) patients and HBV carriers were significantly lower than those in control patients(all P<0.05 or 0.01);In contrast,the percentages of CD8+T lymphocytes showed the opposite trend,and there were also statistically significant.HBV replication level had a positive correlation with the CD8+T lymphocytes and negative correlation with the ratio of CD4+/CD8+T lymphocyte in HBeAg negtive patients.However,there was no corelational relationship between the HBV DNA level and the CD3+T,CD4+T,CD8+T and the ratio of CD4+T/CD8+T lymphocytes subpopulations in HBeAg positive patients,nor did with the HBeAg level increased.Conclusion: Cellular immune function decreased and immune dysregulation in various clinical types of chronic HBV infected patients.HBV DNA level is positively correlated with the cellular immune status in the patients with HBeAg negative chronic hepatitis B.