外周血单个核细胞TNF-α 基因表达与哮喘相关表型*

目的:探讨外周血单个核细胞(PBMC)TNF-α基因mRNA表达与哮喘严重程度、临床病理及相关影响因素的关系。方法:采用实时荧光定量PCR技术检测67例哮喘病人和25例健康对照PBMC中TNF-α mRNA表达水平,分析其与哮喘控制程度、血浆TNF-α浓度、嗜酸性粒细胞百分比(EOS%)、血浆总IgE浓度和哮喘相关影响因素的关系。结果:经方差分析和SNK-q检验,哮喘未控制组PBMC中TNF-α mRNA表达水平高于正常组(P<0.01)、控制组(P<0.01)和部分控制组(P<0.05)。相关分析显示哮喘病人TNF-α mRNA表达与血浆TNF-α浓度和EOS%呈正相关,相关系数分别为r=0.584(P<0.01)和r=0.29(P<0.05),有吸烟史的哮喘病人TNF-α mRNA表达水平高于非吸烟病人(P<0.05)。结论:哮喘病人PBMC中TNF-α mRNA表达水平与哮喘的控制程度呈负相关,与血浆TNF-α浓度呈正相关,PBMC中TNF-α mRNA表达水平与血浆TNF-α浓度可作为哮喘控制程度的参考指标。

The Expression Levels of TNF-mRNA in Peripheral Blood Mononuclear Cells with Asthma-related Phenotypes

Objective: To observe the association of TNF-mRNA expression in peripheral blood mononuclear cells(PBMC) with asthma severity,clinicopathology and asthma-related infuence factors.Methods: The relative expression levels of TNF-mRNA in PBMC were detected by real time fluorescence quantitive PCR from 67 asthma patients with diverse severities and 25 healthy controls.The results were compared among groups and the correlation analysis were performed between the expression levels of TNF-mRNA in PBMC with the levels of plasma TNF-,the percentage of peripheral blood eosinophils(EOS%),the levels of total plasma IgE,and asthma-related influence factors.Results: The relative expression levels of TNF-mRNA in PBMC in uncontrolled asthma patients were higher than that in healthy controls(P < 0.01),controlled asthma patients(P < 0.01),and partly controlled asthma patients(P < 0.05).Pearson product-moment correlation analysis showed that TNF-mRNA expression in PBMC were correlated with plasma TNF-and EOS% with r = 0.584(P < 0.01) and r = 0.29(P < 0.05) respectively.The relative expression levels of TNF-mRNA in patients with positive smoking history were higher than those in patients with no smoking history.Conclusion: The relative expression levels of TNF-mRNA in PBMC were negatively correlated to the levels of asthma control,and were positively correlated to plasma TNF-.Both the expression levels of TNF-mRNA in PBMC and plasma TNF-could be potential makers for management of asthma.