重度子痫前期患者外周血NK 细胞功能的研究*

目的:探讨重度子痫前期患者外周血中NK细胞数量、杀伤功能以及相关细胞因子的变化。方法:选择重度子痫前期患者25例(研究组)以及正常妊娠妇女25例(对照组),流式细胞术测定外周血中NK细胞数量,细胞毒实验测定NK细胞的杀伤活性,ELISA法测定血清IFN-γ及IL-2的浓度。结果:研究组患者外周血中NK细胞的数量以及杀伤活性显著高于对照组;研究组外周血中IFN-γ、IL-2的浓度也显著高于对照组。结论:重度子痫前期患者外周血NK细胞数量增多,杀伤活性增强,血清中NK细胞相关细胞因子也增加。这些改变可能参与重度子痫前期的发病机制。

Research on Peripheral Blood NK Cell Function in Severe Pre-Eclamptic Patients*

Objective: To explore the changes of numbers and cytotoxic activity of NK cell and related cytokines in peripheral blood of severe pre-eclamptic patients.Methods: 25 severe pre-eclamptic patients(study group) and 25 normal pregnant women(control group) were included in this study.The rates of NK cell in peripheral blood were measured by flow cytometry.The cytotoxic activity of NK cell was detected by cytotoxicity assay.The concentrations of serum IFN-γ and IL-2 were determined by ELISA.Results: The rate and cytotoxic activity of peripheral blood NK cell of study group was significantly higher than that of control group.The serum IFN-γ and IL-2 levels of study group were also significantly higher than that of control group.Conclusion: The peripheral blood NK cell number,cytotoxic activity and related cytokines all increased in severe pre-eclamptic patients.These changes may play an important role in the cause of severe pre-eclampsia.