梓醇对大鼠脑缺血再灌注损伤的保护作用研究

目的:探讨梓醇对缺血再灌注大鼠脑损伤后的保护作用.方法:采用传统大脑中动脉阻塞(MCAO)方法制备大鼠局灶性缺血模型,根据随机数字表法将SD大鼠分为MCAO组、对照组(vehicle组)及梓醇处理组(catalpol组),缺血再灌注48 h后观察各组大鼠神经功能学评分和脑梗死容积.分别于术前、术后6h、24 h、48 h取大鼠脑组织样本,检测匀浆中谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)的变化情况.结果:与vehicle组和MCAO组相比,catalpol处理组神经功能学评分降低(P＜0.05)；其梗死容积较小(P＜0.05).组织匀浆结果显示catalpol处理组脑匀浆中GSH-PX活力升高,MDA含量下降(P＜0.05).结论:梓醇可能通过降低脑内自由基水平、控制脂质过氧化程度,对缺血再灌注引起的大鼠脑损伤产生神经保护作用.

Protective Effect of Catalpol on Cerebral Ischemia Reperfusion Injury in Rats

Objective: To investigate the effect of catalpol on the impact of cerebral ischemia-reperfusion injury in rats.Methods: Cerebral ischemia-reperfusion model was established by middle cerebral artery occlusion(MCAO).SD rats were randomly divided into MCAO groups,vehicle group and catalpol group.Neurological dysfunction scores(NDS) and the infarct volume were measured at 48h after ischemia-reperfusion.The concentration of glutathione peroxidase(GSH-PX) and maleic diadehyde(MDA) in brain plasm were measured at 6h,24h,48h pre-operation and after ischemia-reperfusion.Results: The result of NDS showed that catalpol group was better than that of the MCAO and the vehicle group(P<0.05);The volume of brain infarct in catalpol group was significantly smaller than that of the MCAO and the vehicle group(P<0.05);The activity of GSH-PX increased but the concentration of MDA decreased in brain plasm(P<0.05).Conclusion:The catalpol could increase the antiperoxidase activity and decrease oxygen free radicals to protect the focal cerebral ischemic injury in rats.