人胃粘膜乳酸杆菌与幽门螺杆菌感染的相关性

目的:探讨胃内正常菌群乳酸杆菌对幽门螺杆菌(Helicobacter pylori,HP)的影响。方法:51例胃粘膜活检标本均取自于行胃镜检查的胃炎患者,分离培养乳酸杆菌,通过扩增其16S rRNA基因并测序来鉴定乳酸杆菌的种类;胃炎程度及活动度的分类依据悉尼分类系统,运用改良Gimesa染色鉴定HP感染。结果:胃粘膜中共分离出9种乳酸杆菌,分离阳性率为49.0%;乳酸杆菌阳性病人与阴性病人的HP感染率、胃炎程度的差异及胃炎活动度的差异均无统计学意义(P>0.05);HP阳性病人的胃炎程度较HP阴性病人更严重(P<0.05);有益生菌作用的乳酸杆菌与非益生菌类乳酸杆菌的HP感染率差异无统计学意义(P>0.05)。结论:胃内乳酸杆菌的存在对HP感染无影响。

Association of Lactobacilli in Human Gastric Mucosa with the Helicobacter Pylori infection

Objective: To investigate the influence of Lactobacilli of the normal microbiota in the human stomach on the infection of Helicobacter pylori(H.pylori).Methods: A total of 51 specimens of gastric mucous were collected from gastritis patients undergoing routine endoscopy.Lactobacilli were isolated using Rogosa Agra plates.The identification of species of the bacterium was conducted using 16S rRNA gene sequencing.The degree and activity of gastritis were evaluated according to Sydney System.H.pylori detected with Gimesa staining.Results: A total of 9 species of lactobacilli were isolated from the stomach.The prevalence of lactobacilli was 49.0 %.There were no significant differences in the H.pylori infection rate,the degree and activity of the inflammation between the lactobacillus positive patients and lactobacillus negative patients(P> 0.05),however,H.pylori positive patients had a more severe inflammation compared to HP-negative patients(P <0.05).There were no differences in the prevalence of H.Pylori infection between the probiotic species of lactobacilli positive and negative patients(P> 0.05).Conclusion: It appeared that the presence of species of lactobacilli in the stomach had no influence on the H.pylori infection.