辐射所致小鼠肾脏氧化损伤及川芎嗪的保护作用

目的:研究川芎嗪对辐射所致小鼠肾脏氧化损伤的预防和治疗作用。方法:采用60Co-γ射线5 Gy全身单次照射小鼠造模,在照射前和照射后分别于每天腹腔注射川芎嗪130 mg/kg,连续给药10 d,进行预防和治疗,并设对照组,观察肾组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)及总抗氧化力(T-AOC)的变化。结果:与阴性对照组比较,照射可显著增加肾组织中MDA的含量(P<0.05),降低SOD、CAT的活性(P<0.05),升高GSH-Px活性(P<0.05),降低GSH含量(P<0.05),使肾组织T-AOC下降(P<0.05),。与照射组比较,给予川芎嗪预防和治疗后,均可降低肾组织MDA含量(P<0.05),升高肾组织T-AOC(P<0.05),且治疗组优于预防组,与阴性对照组无显著性差异。同时,预防组可使SOD活性和GSH含量升高(P<0.05),治疗组可使SOD和CAT活性增高(P<0.05),但均对GSH-Px活性无显著影响(P>0.05)。结论:川芎嗪具有很好的抗氧化作用,无论预防和治疗均可降低辐射所致小鼠肾脏的氧化应激损伤,并且治疗效果优于预防效果。

Oxidative Stress Damage Induced by Irradiation in Mouse Kidney and the Protective Effect of Ligustrazine against it

Objective: To investigate the oxidative stress damage induced by irradiation in mouse kidney and the preventive and therapeutic effect of Ligustrazine against it.Methods: Mice were exposed to 5Gy single 60Co-irradiation to induce oxidative stress,the concentration of Ligustrazine for intraperitoneal is 130 mg/kg to prevent and treat liver injury before and after irradiation,The injection lasted 10 days.The parameters of malondialdehyde(MDA),superoxide dismutase enzyme(SOD),catalase(CAT),reduced glutathione hormone(GSH),glutathione peroxidase(GPx),total antioxidation capability(T-AOC)activity in kidney were measured.Results: Com-pared with that in the normal control group,irradiation significantly increased kidney content of MDA(P<0.05),decreased CAT,SOD activity(P<0.05),GSH-Px activity(P<0.05),while it decreased kidney content of GSH(P<0.05),the kidney tissue T-AOC decreased(P<0.05).By use of Ligustrazine,MDA content decreased(P<0.05),T-AOC were significantly higher(P<0.05),CAT activity increased(P<0.05) and the effect of treatment group was better than the prevention group had no significant difference(P<0.05) compared with that in the normal group.Meanwhile,the activity of SOD and the content of GSH significant increased in prevention group(P<0.05);The ac-tivity of SOD and CAT significant increased in treatment group(P<0.05);But GSH-Px activity was no significant difference either in treatment group or in prevention group.Conclusion: The irradiation can cause oxidative stress damage in mouse kidney tissue,Ligus-trazine can reduce or reverse this damage both in treatment and prophylaxis,and treatment group is better than the prevention group.