YKL-40 和NF-κ B 在子宫内膜异位症中的表达及相关性研究

目的:通过检测人类软骨蛋白39(YKL-40)和核转录因子KappaB(NF-κB)在子宫内膜异位症组织中的表达,探讨二者与子宫内膜异位症的关系及二者的相关性。方法:用免疫组化二步法检测40例子宫内膜异位症(EMS)患者的异位内膜和在位内膜及40例子宫肌瘤患者子宫内膜(对照组)YKL-40和NF-κB的表达,并对检测结果进行统计学分析。结果:YKL-40、NF-κB在子宫内膜异位症患者的异位和在位内膜及对照组内膜中的表达率分别为67.5%、62.5%、35%及88.5%、57.5%、32.5%,差异有统计学意义(P<0.01);在不同月经周期YKL-40表达无统计学意义;在位内膜和正常内膜NF-κB的表达分泌期高于增殖期,差异有统计学意义(P<0.05);YKL-40和NF-κB在三种内膜中的表达具有正相关性,相关系数分别是0.305,0.267和0.457(P<0.01)。结论:YKL-40和NF-κB在EMS发生发展中起重要的作用。

Expressions and Correlation of YKL-40 and Nuclear Factor- kappaB in Endometriosis

Objective: To investigate the expressions of Human cartilage glycoprotein-39(YKL-40) and Nuclear factor-kappaB in endometriosis,and to evaluate the relationship between the YKL-40 and NF-κ B expressions.Methods: Immunohistochemistry was used to examine the YKL-40 and NF-κ B protein expressions in eutopic(n=40) and ectopic(n=40) endometrium of Ems,as well as endometrium of hystromyoma(n=40).The results were analyzed statistically.Results: The positive rate of YKL-40 and NF-κ B expressions in ectopic,eutopic and normal endometrium respectively were 67.5%,62.5%,35% and 88.5%,57.5%,32.5%(P<0.01);NF-κ B expression was higher in secretory phase than that in proliferative phase in the eutopic and normal endometrium(P<0.05),but YKL-40 was not correlated with the phase of menstrual cycle;Furthemore,the expression of YKL-40 and NF-κ B protein was positively correlated in these three medometrial tissue(rs=0.305,0.247 and 0.467,P<0.01).Conclusion: The over expression of YKL-40 and NF-κ B may play an important role in the pathogenesis of endometriosis.