一株红树林真菌抗单纯疱疹病毒Ⅱ型的研究

目的:对分离自海南红树林真菌菌株PH0016的胞外多糖(exopolysaccharide,EPS)抗单纯疱疹病毒Ⅱ型(Herpes simplex virus type 2,HSV-2)活性进行研究。方法:收集纯化菌株PH0016产生的EPS,体外采用细胞病变观察EPS的细胞毒性和抗HSV-2作用。为观察EPS对HSV-2体内感染的治疗效果,小鼠颅内注射0.02ml滴度为100LD50/0.1ml-1的HSV-2,24小时后以不同浓度EPS灌胃,持续7天,14天后计算小鼠存活率和存活时间。菌株分类采用形态学观察和ITS序列分析。结果:EPS可抑制HSV-2病毒活性,病毒感染的细胞病变的半抑制浓度(Inhibited concentration,IC50)为313μg/mL,SI为11.43。EPS 25mg.kg-1.d-1灌胃后,小鼠存活率为40.0%,存活时间为9.82±1.87天,相比模型组实验动物存活时间和存活率均有提高。菌株初步鉴定为淡紫色拟青霉。结论:从海南红树林分离一株淡紫色拟青霉,其产生的EPS具有抗HSV-2活性,值得进一步研究。

A Study on Anti Herpes Simplex Virus Type 2 Activities of one Fungi Strain Isolated from Mangrove

Objective: The antiviral activities of exopolysaccharide(EPS)from one kind of fungi strain PH0016 isolated from mangrove in Hainan province against HSV-2 was explored.Methods: The EPS of strain PH0016 was collected and purified.The toxicity and antiviral actions of EPS in vitro was evaluated by observing cytotoxicity effect and cytopathic effect(CPE).To study the therapeutic effect of EPS on HSV-2 infection in vivo,every mouse was injected intracerebrally 0.02ml HSV-2 solution with the titer of 100LD50 0.1ml-1.After 24hours,the mice were treated by EPS with different doses respectively for 7 days.Surviaval rate and survival time of mice were observed after 14 days.The taxonomic status of strain was identified by means of morphological observations and analysis of the ITS sequence.Results: The EPS inhibited HSV-2 at the IC50 values of 313μg/ml,and SI values of 11.43 in vitro.After using EPS 25mg·kg-1·d-1,the survival rate of the mice infected with HSV-2 was 40.0%.The average survival time was 9.82±1.87 days.The mortality of the infected mice was reduced and the survival days prolonged.The strain was identified as Paecilomyces lilacinus.Conclusion: EPS from fungi strain PH0016 isolated from mangrove in Hainan province had remarkable anti HSV-2 activity,this EPS could be utilized for the further research.