平喘固本合剂对昆系小鼠急性哮喘模型气道炎症作用影响

目的:探讨平喘固本合剂对昆系小鼠急性哮喘模型气道炎症作用影响及相关机制。方法:昆明系小鼠40只,随机分为对照组(A),哮喘模型组(B),喘固本合剂治疗组(C),布地奈德雾化治疗组(D)及平喘固本合剂联合布地奈德雾化治疗组(E)。用鸡卵蛋白(OVA)致敏建立昆系小鼠哮喘急性气道炎症模型,并给与药物治疗10天。对各组支气管肺泡灌洗液(BALF)中各种细胞进行分类并计数,观察肺组织的病理变化,同时应用ELISA法测定灌洗液中炎症相关因子的水平变化。结果:与B组比较,C组、D组、E组BALF中细胞总数、嗜酸性粒细胞数、巨噬细胞数及IL-4、IL-4/IFN-γ明显降低(P<0.05),IFN-γ明显升高(P<0.05)。对于IL-4、IL-4/IFN-γ水平的比较C组与D组无明显统计学差异,E组与两者具有明显统计学意义(P<0.05)。HE染色显示C组、D组、E组较单纯模型组炎症细胞浸润,平滑肌肥厚及黏膜肺组织水肿等炎症表现明显减轻。结论:平喘固本合剂对哮喘昆系小鼠气道炎症有明显的抑制作用,其作用机制可能与抑制IL-4的表达、炎性细胞聚积及促进IFN-γ的表达有关,并且可能与糖皮质激素有一定协同作用。

Effect of Pingchuan-Guben Decoction on Acute Airway Inflammation in Kuming Mouse Model of Asthma

Objective: To investigate the effect of Pingchuan-Guben Decoction on acute airway inflammation of asthma and relate mechanism in mouse model of asthma.Methods: Forty Kunming mice were randomly divided into five groups averagely: negative control group(A),asthma model group(B),Pingchuan-Guben Decoction group(C),Budesonide group(D),Pingchuan-Guben Decoction + Budesonide group(E).10 days after therapy on OVA challenged asthma model,total and differential cells were counted from bronchoalveolar lavage fluid(BALF),the levels interleukin-4(IL-4) and Interferon-γ(IFN-γ) in BALF were measured by ELISA.Results: Total cells countings,eosinophils,macrophages and IL-4,IL-4/IFN-γ in BALF of group C,D,E were significantly lower than that in group B(P<0.05),IFN-γ was higher than that in group B(P<0.05).The level of IL-4,IL-4/IFN-γ had no significant difference between the group C and group D(P > 0.05),while the level of IL-4,IL-4/IFN-γ in the group E were significantly lower(P<0.05).HE staining showed that compared with those in the treatment groups,there were a large number of inflammatory cells infiltration,heavier smooth muscle hypertrophy and mucous membrane hyperemia in the asthma group.Conclusions: Pingchuan-Guben Decoction may potentially inhibit airway inflammation of asthma by inhibiting of IL-4 expression,inflammatory cells accumulation and promoting the expression of IFN-γ.Pingchuan-Guben composition may have synergistic effect with glucocorticoid hormones.