高危HPV 感染及hTERC 基因在宫颈癌发生发展中的相关性

目的:探讨高危人乳头瘤病毒及人类染色体端粒酶基因(hTERC)在宫颈癌发生发展中的关系。方法:2010年10月至2011年05月就诊于哈尔滨医科大学附属第一医院妇科患者445例,采用表面等离子体谐振法(SPR)及荧光原位杂交法(FISH)分别检测高危HPV和hTERC的表达情况,对任一结果阳性者行阴道镜下宫颈活检和病理学检查,其中炎症对照组45例,CIN组33例及宫颈癌组6例。结果:单一型HPV29例,多重型26例,最常见为HPV16型合并的二型感染(17例)。在炎症组,CINⅠ组,CINⅡ组、CINⅢ组及宫颈癌组中多重高危HPV比率为17.78%、28.57%、44.44%、50.00%、66.67%,与炎症对照组比较其他组均有统计学意义(P<0.05)。hTERC基因在各组中的阳性表达率分别为2.22%、14.29%、61.11%、75.00%及100%。CIN组及宫颈癌的hTERC基因表达与炎症组比较差异均有统计学意义(P均<0.01),其中CINⅡ及以上各组差异明显。多重HPV感染与hTERC表达呈中等正相关(r=0.462,P<0.01)。结论:随着宫颈病理类型分级升高,HPV感染及hTERC基因表达率升高,hTERC基因在宫颈癌发生发展中起重要作用;高危HPV感染与hTERC基因的异常扩增关系密切,提示二者可能存在因果关系。

Correlation of High Risk-HPV Infection and the Human Telomerase Gene in Cervical Cancer

Objective: To investigate the relationship of HR-HPV infection and the Human Telomerase Gene(hTERC) in the generation and progression of cervical cancer.Methods: The cervical samples of 445 women visited the First Clinical College of Harbin Medical University from October 2010 to May 2011 underwent the test for HR-HPV with SPR method.hTERC expression was detected by FISH.Then the sample with any positive result underwent colposcopic indicated biopsy and pathological diagnosis.Results: The pathological results showed that 45 of inflammation,33of CIN and 6 Cervical cancer.Among the 445 cases,there were 29 of single HR-HPV infection and 26 of multi-infection,including HPV 16/X double infection(17/26).The rate of multiple HR-HPV infection was 66.67% in cervical cancer group,28.57% in CINⅠgroup,44.44% in CINⅡgroup,50.00% in CINⅢ group and 30.56% in inflammation group,respectively.The infection rate of any group was higher than that in inflammation group(all P<0.05).The positive amplification rate of hTERC in inflammation group was 2.22%,while it was 14.29% for CINⅠ,61.11% for CINⅡ,75.00% for CINⅢand the cervical 100%for cancer,which showed a significant difference(all P<0.01),evidently in CINⅡand CINⅡ+.The multiple HR-HPV infection was middle-positive correlated to hTERC expression(r=0.462,P<0.01).Conclusions: As the development of cervical cancer,the HR-HPV Infection and hTERC expression increased and hTERC gene may play an important part in the pathogenesis of cervical cancer.The HR-HPV infection may be a positive-stimulated fact of hTERC gene.