Identification,characterization, and comparison of RNA-degrading enzymes of wheat and barley |
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Authors: | Yang Yen P Stephen Baenziger |
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Institution: | (1) Department of Agronomy, University of Nebraska, P.O. Box 830915, 68583-0915 Lincoln, Nebraska |
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Abstract: | RNA-degrading enzymes play an important role in regulating gene expression, and sequence analyses have revealed significant
homology among several plant RNA-degrading enzymes. In this study we surveyed crude extracts of the above-ground part of the
common wheat (Triticum aestivum L.) and the cultivated barley (Hordeum vulgare L.) for major RNA-degrading enzymes using a substrate-based SDS-PAGE assay. Fifteen wheat and fourteen barley RNA-degrading
enzymes, with apparent molecular masses ranging from 16.3 to 40.1 kD, were identified. These RNA-degrading enzymes were characterized
by their response to pH changes and addition of EDTA and ZnCl2 to the preincubation or incubation buffers. The 33.2- to 40.1-kD wheat and barley, 31.7-kD wheat, and 32.0-kD barley enzyme
activities were inhibited by both zinc and EDTA and were relatively tolerant to alkaline environment. The 22.7- to 28.2-kD
enzymes were inhibited by zinc but stimulated by EDTA. The 18.8-kD enzyme exists in both wheat and barley. It was active in
an acid environment, was inhibited by zinc, but was not affected by EDTA. Two enzyme activities (31.0 and 32.0 kD) are unique
to the common wheat.
Contribution from Agriculture Research Division, University of Nebraska, Journal Series No. 9895. |
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Keywords: | Triticum aestivum L Hordeum vulgare L RNase DNase phylogenesis |
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