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| 酶法破碎裂殖壶菌提取胞内油脂 | |
| 引用本文： | 吕浩,崔丁维,胡学超,张红漫.酶法破碎裂殖壶菌提取胞内油脂[J].生物加工过程,2011,9(3):27-30. |
| 作者姓名： | 吕浩 崔丁维 胡学超 张红漫 |
| 作者单位： | 1. 南京工业大学,生物与制药工程学院,南京,210009 2. 江苏省工业生物技术创新中心,南京,211816 3. 南京工业大学,理学院,南京,210009 |
| 基金项目： | 国家自然科学基金重点资助项目，国家自然科学基金资助项目，国家科技支撑计划资助项目 |
| 摘 要： | 采用酶法破碎裂殖壶菌提取胞内油脂,进行单因素实验和正交实验优化酶解反应条件,酶解反应的影响因素主次顺序依次为酶用量、温度、时间、pH,最佳酶解工艺参数:55 ℃、pH 9.5、搅拌反应2.5 h、酶用量为菌体生物量的2%.在该条件下,胞内油脂的提取量高达(81.53±0.33) g/L,过氧化值仅为0.15,酸价为0.24. |
| 关 键 词： | 裂殖壶菌 酶法 水解 提油量 |
Extraction of oil from Schizochytrium sp.by enzymatic hydrolysis |
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| L Hao,CUI Dingwei,HU Xuechao,ZHANG Hongman.Extraction of oil from Schizochytrium sp.by enzymatic hydrolysis[J].Chinese Journal of Bioprocess Engineering,2011,9(3):27-30. | |
| Authors: | L Hao CUI Dingwei HU Xuechao ZHANG Hongman |
| Institution: | L(U) Hao,CUI Dingwei,HU Xuechao,ZHANG Hongman |
| Abstract: | The oil extraction process of Schizochytrium sp.was optimized by enzymatic hydrolysis.By single factor and orthogonal experimental tests,the priority orders of the factors influencing the enzymatic hydrolysis were enzyme concentration,temperature,time,and pH.The optimum conditions were hydrolysis time 2.5 h,temperature 55 ℃,pH 9.5,and adding 2% of enzyme.The conditions resulted in the oil extraction yield up to（81.53±0.33） g/L,peroxide value and acid value were 0.15 and 0.24,respectively. |
| Keywords: | Schizochytrium sp enzymatic hydrolysis oil extraction yield |
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