Extraction of DNA from milligram amounts of fresh,herbarium and mummified plant tissues |
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| Authors: | Scott O Rogers Arnold J Bendich |
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| Institution: | (1) Department of Botany, University of Washington, 98195 Seattle, WA, U.S.A.;(2) Department of Botany and Genetics, University of Washington, 98195 Seattle, WA, U.S.A. |
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| Abstract: | Summary We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3–200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22–118 years old) and mummified seeds and embryos (500 to greater than 44 600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI. |
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| Keywords: | ,cetyltrimethylammonium bromide,CTAB |
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