Chemical stabilization of recombinant horseradish peroxidase |
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| Authors: | Anne Marie O'brien Ciarán Ó Fágáin |
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| Affiliation: | (1) School of Biological Sciences, Dublin City University, 9 Dublin, Ireland |
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| Abstract: | Summary Unglycosylated recombinant horseradish peroxidase (HRP C*) had a half life of 21 minutes at 65°C compared with only 5 minutes for the plant enzyme (HRP). The half life of HRP C* at 65°C increased by 5-fold following modification with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Tolerance to 60% 1,4-dioxan also increased whilst tolerance to 30% dimethylformamide was unchanged. |
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