The in vitro anti-platelet,antioxidant and cellular immunity activity of Phellinus gilvus fractional extracts |
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Authors: | Z. Q. Chang M. H. Hwang M. H. Rhee K. S. Kim J. C. Kim S. P. Lee W. S. Jo S. C. Park |
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Affiliation: | (1) College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea;(2) College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757, Republic of Korea;(3) The Center for Traditional Microorganism Resources, Keimyung University, Daegu, 704-701, Republic of Korea;(4) Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu, 702-320, Republic of Korea |
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Abstract: | The in vitro anti-platelet and antioxidant activities of various solvent extracts from Phellinus gilvus (PG), and the effects of hot water extract from PG (PGW) on murine cellular immunity were investigated. Chloroform extract (CE), methanol extract (ME) and butanol extract (BE) from PG could significantly inhibit platelet aggregation induced by thrombin. Ethyl acetate extract (EAE), BE, ME from PG had significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity compared with the control, and the EAE showed the highest effect with IC50 values of 13.34 μg/ml, which is higher than that of ascorbic acid (40 μg/ml). In addition, EAE displayed the inhibition of xanthine oxidase (XO) activity with IC50 value of 2.45 μg/ml. As to the cellular immunity activity, PGW could enhance both the lipopolysaccharide (LPS)-induced B lymphocyte proliferation and concanavalin A (Con A)-induced T lymphocyte proliferation in vitro. The phagocytosis of both peritoneal macrophages and RAW264.7 macrophage cells were also increased by the addition of PGW. Moreover, PGW was found to inhibit the nitric oxide (NO) production of RAW264.7 macrophages induced by LPS in a concentration-dependant manner. |
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Keywords: | Phellinus gilvus Platelet aggregation Antioxidant Immunomodulating activity Nitric oxide |
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