Pyridoxal kinase immunoreactivity in rabbit brain |
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| Authors: | Kivoshi Ohkawa Tae Hirakawa-Sakurai Kensuke Joh Tadashi Asakura Koji Takada Makoto Matsuda |
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| Institution: | (1) Department of Biochemistry, Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-Ku, Tokyo 105, Japan;(2) Department of Pathology, Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-Ku, Tokyo 105, Japan;(3) Biomedical Division, SRL, Tokyo |
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| Abstract: | Murine polyclonal antibody against purified bovine brain pyridoxal kinase (EC 2.7.1.35) was generated and showed cross-reactivity with rabbit brain pyridoxal kinase. This antibody was used to immunohistochemically examine the distribution of pyridoxal kinase in the rabbit brain. The cytoplasm of neuronal cells and neuroglial cells in the cerebral cortex, hippocampal region, brain nuclei and cerebellar cortex showed positive staining with various degrees of intensity. The neuronal cells and surrounding fibers in some brain nuclei, such as the area tegmentalis ventralis or the substantia nigra, showed intense staining. The neuronal cells of the hippocampal region showed somewhat weak reactivity, but some with intense reactivity were found sparsely distributed and positive staining fiber networks of a very low density were also observed. |
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| Keywords: | Pyridoxal kinase anti-pyridoxal kinase antibody vitamin B6 immunohistochemistry |
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