Bioreactor operating strategy inThalictrum rugosum plant cell culture for the production of berberine |
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Authors: | Jeong-Woo Choi Young-Kee Kim Won Hong Lee Henrik Pedersen Chee-Kok Chin |
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Institution: | (1) Department of Chemical Engineering, Sogang University, C.P.O.Box 1142, Seoul, Korea;(2) Department of Chemical and Biochemical Engineering, Rutgers University, P.O. Box 909, 08855 Piscataway, New Jersey, U.S.A.;(3) Department of Plant Science, Rutgers University, P.O. Box 231, 08903 New Brunswick, New Jersey, U.S.A. |
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Abstract: | Optimal substrate feeding strategy in bioreactor operation was investigated to increase the production of secondary metabolite
in a high density culture of plant cell. It was accomplished by the previously proposed structured kinetic model that describes
the cell growth and synthesis of the secondary metabolite, berberine, in a batch suspension culture ofThalictrum rugosum. Four types of operation strategies for sugar feeding intoT. rugosum culture were proposed based on the model, which were the periodic fedbatch operations to maintain the cell activity, the
cell viability, and the specific production rate, and the perfusion operation to maintain the specific production rate. From
the simulation results of these strategies, it could be found that the periodic fed-batch operation and the perfusion operation
could achieve the higher volumetric production of berberine (mg berberine/L) and specific production yield (mg berberine/g
dry cell weight) than those of batch cultures. Although the highest productivity (mg berberine/day) of berberine could be
achieved by the periodic fed-batch operation to maintain the cell activity compared with the other strategies in the periodic
fed-batch operations, the specific production yield was low due to the higher maximum dry cell weight than other cases. The
periodic fed-batch operation to maintain cell viability resulted in the highest volumetric production of berberine and specific
production yield compared with the other strategies. In the cases of maintaining the specific production rate, the per-formance
of the periodic fed-batch operation was better than that of the perfusion operation in the respect of the volumetric production
and productivity of berberine. In order to increase the volumetric production of berberine and to get the highest specific
production yield, the periodic fed-batch operation to maintain cell viability could be chosen as the optimal operating strategy
in high density, culture ofT. rugosum plant cell. |
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Keywords: | structured kinetic model Thalictrum rugosum high density plant cell culture fed-batch operation perfusion operation operation strategy |
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