In vitro storage of cedar shoot cultures under minimal growth conditions |
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Authors: | Begoña Renau-Morata Isabel Arrillaga Juan Segura |
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Institution: | (1) Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100-Burjasot Valencia, Spain |
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Abstract: | We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4°C and reduced light intensity, provided that they were grown on a diluted modified MS medium. The addition of 6% mannitol to the storage media affected negatively survival and multiplication capacity of the cultures. The slow-growth storage conditions used in our experiments did not induce remarkable effects on both RAPD variability and average DNA methylation in the species. |
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Keywords: | Cedrus DNA methylation In vitro storage Minimal growth RAPD |
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