Constitutively activated barley ROPs modulate epidermal cell size, defense reactions and interactions with fungal leaf pathogens |
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Authors: | Indira Priyadarshini Pathuri Nina Zellerhoff Ulrich Schaffrath Götz Hensel Jochen Kumlehn Karl-Heinz Kogel Ruth Eichmann Ralph Hückelhoven |
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Institution: | 1. Lehrstuhl für Phytopathologie, Technische Universit?t München, Am Hochanger 2, 85350, Freising, Germany 2. Institute for Biology III (Plant Physiology), RWTH Aachen, 52056, Aachen, Germany 3. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstra?e 3, 06466, Gatersleben, Germany 4. Institute of Phytopathology and Applied Zoology, University of Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany
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Abstract: | RHO-like monomeric G-proteins of plants (ROPs, also called RACs), are involved in plant development and interaction with the
environment. The barley (Hordeum vulgare) ROP protein HvRACB has been shown to be required for entry of the biotrophic powdery mildew fungus Blumeria graminis f.sp. hordei (Bgh) into living host cells. To get a deeper insight into evolutionarily conserved functions of ROPs in cell polarity and pathogen
responses, we stably expressed constitutively activated (CA) mutant variants of different barley ROPs (HvRACB, HvRAC1, HvRAC3)
in barley. CA HvROPs induced epidermal cell expansion and/or abolished polarity in tip growing root hairs. All three CA HvROPs
enhanced susceptibility of barley to penetration by Bgh whereas only CA HvRAC1 supported whole cell H2O2 production in non-penetrated cells. Despite increasing penetration by Bgh, CA HvRAC1 promoted callose deposition at sites of fungal attack and resistance to penetration by Magnaporthe oryzae. The data show an involvement of ROPs in polar growth processes of the monocot barley and in responses to fungal pathogens
with different life style. |
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Keywords: | Blumeria Cell size GTPase ROP Susceptibility |
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