Proteomic analysis of differentially accumulated proteins during ripening and in response to 1-MCP in papaya fruit |
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Authors: | Huerta-Ocampo José Ángel Osuna-Castro Juan Alberto Lino-López Gisela Jareth Barrera-Pacheco Alberto Mendoza-Hernández Guillermo De León-Rodríguez Antonio Barba de la Rosa Ana Paulina |
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Institution: | IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José No. 2055, Lomas 4a sección, 78216 San Luis Potosí, SLP, Mexico. |
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Abstract: | Papaya (Carica papaya L.) is a climacteric fruit susceptible to postharvest losses due to the ethylene-induced ripening. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), has been used worldwide as a safe postharvest non-toxic agent, but the physiological and biochemical modifications induced by 1-MCP are not well understood. Using the 2-DE analysis, we report the changes in the protein profiles after 6 and 18 days of postharvest and the effect of the effect of 1-MCP treatment on fruits. Twenty seven protein spots showing differences in abundance during ripening were successfully identified by nano-LC-ESI/MS/MS. Some spots corresponded to the cell wall degrading enzymes related to fruit ripening; others were involved in oxidative damage protection, protein folding, and cell growth and survival that were induced by 1-MCP. This is the first proteomic report analyzing the effect of 1-MCP in papaya ripening. The present data will help to shed light on papaya fruit ripening process. |
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