Establishment of functional epithelial cell lines from a rat thymoma and rat thymus |
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Authors: | Akiramasuda Kenzo Ohtsuka Mutsushi Matsuyama |
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Institution: | (1) Laboratory of Ultrastructure Research, Aichi Cancer Center Research Institute, Chikusa-ku, 464 Nagoya, Japan;(2) Department of Experimental Radiology, Aichi Cancer Center Research Institute, Chikusa-ku, 464 Nagoya, Japan;(3) Present address: Department of Pathology, Nagoya University School of Medicine, Showa-ku, 466 Nagoya, Japan |
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Abstract: | Summary Seven clonal epithelial cell lines from a thymoma of an (ACI/NMs×BUF/Mna)F1 rat and seven clonal epithelial cell lines from
an ACI/NMs rat thymus were established in a medium containing 1 μM dexamethasome (DM) and were characterized cytologically. Long-term treatment of DM stabilized the epithelial nature of these
epithelial cells irreversibly. The established cell lines showed a polygonal shape, were positively stained with antikeratin
antiserum and had tonofilaments and desmosomes. Species of their keratin paptides were the same as those of normal thymic
epithelial cells in primary cultures. The cell lines were positively stained with Th-4 monoclonal antibody which preferentially
stains the medullary epithelial cells of the thymus, but not with Th-3 which preferentially stains the subcapsular and cortical
epithelial cells of the thymus. The cells from the rat thymoma were much large than those from the normal thymus, as reflected
in their primary cultures. No transformed phenotypes, such as high growth rate, high saturation density anchorage independency,
low serum dependency and so on, were found on the cell lines from the thymoma as in the cell lines from the normal thymus
by in vitro assays. DNA synthesis of the thymic lymphocytes was stimulated by culturing with a line of rat thymoma with no
lectins. Thymic lymphocytes strongly bound on the cell lines from the thymoma and changed the shape of the cells. These cell
lines may be useful to investigate the mechanism of thymomegenesis and the interactions between epithelial cells and thymocytes
in the rat thymoma. |
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Keywords: | thymoma epithelial cells cell lines dexamethasone |
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