两株产生免疫抑制剂的小双孢菌分离株的鉴定

从江苏无锡土壤中分离到两株玫瑰小双孢菌SIPI226和SIPI207，经形态、化学分析、Ribotyping及16S rRNA分析，两菌株细胞壁含meso\|DAP、磷酸类脂PIV、无枝菌酸，醌为MK9(H0,H2,H4)，G+C mol%分别为683和694。经初步鉴定为玫瑰小双孢菌的两个新亚种：玫瑰小双孢菌无锡亚种(Microbispora rosea subsp. wuxiensis)和玫瑰小双孢菌鼋头渚亚种(Microbispora rosea subsp. yuantouzhuensis)。菌株SIPI226和SIPI207分别为玫瑰小双孢菌无锡亚种和玫瑰小双孢菌鼋头渚亚种的典型菌株。

CLASSIFICATION FOR TWO ISOLATES OF MICROBISPORA PRODUCING IMMUNOSUPPRESSANTS

Two strains named SIPI\|226 and SIPI\|207 producing immunosuppressants were isolated from soil samples collected in park of Wuxi city in China.Identification was done by morphological observation,chemical analysis and nucleic acid ribotyping and 16S rRNA sequence.Two strains contain meso\|diaminopimelic acid in cell walls,Type PIV phospholipids,a lack of mycolic acids,MK\|9(H 0,H 2,H 4) menaquinones and G C content of 68.3% to 69.4%,and two strains have 97.2% to 98.6% homology in 16S rRNA gene sequences,and the DNA homology by Ribotyping are 53% to 70%,but differ from seven other mesophilic \%Microbispora rosea\% strains.It could be concluded that these two strains represented two new subspecies in the genus of \%Microbispora rosea.\%We designated the strains as Microbispora rosea subsp. wuxiensis and Microbispora rosea subsp. yuantouzhuensis. The type strains of M.rosea subsp. wuxiensis and M.rosea subsp. yuantouzhuensis are SIPI\|226 and SIPI\|207,respectively.